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KMID : 1102020170470030171
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Applied Microscopy
2017 Volume.47 No. 3 p.171 ~ p.175
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Structural Analysis of Exosomes Using Different Types of Electron Microscopy
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Choi Hyo-Sun
Mun Ji-Young
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Abstract
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Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.
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KEYWORD
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Exosome, Electron tomography, Serial block face section through focused ion beam, Cryo-transmission electron microscopy
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